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Identification of two forms of the Eso1 protein in Schizosaccharomyces pombe 期刊论文

编号：

004e697f-934a-4477-8a0e-289659d0ee82
作者：

Chen, Zhiming#*^[1]Cao, Hongshi^[2];Guo, Weichao^[3];Lu, Yingqiang^[1];
语种：

English
期刊：

CELL BIOLOGY INTERNATIONAL ISSN：1065-6995 2014 年 38 卷 5 期 (682 - 688) ; MAY
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关键词：

Schizosaccharomyces pombe Rad30 Eso1 cohesin cohesion TAP purification
摘要：

In Schizosaccharomyces pombe, Eso1p is a protein fusion. Two-thirds of its N-terminus is conserved to budding yeast Rad30, which functions in error-free replication of UV-damaged DNA. A third of the C-terminus is highly conserved to budding yeast Eco1, a lysine acetyltransferase, which is essential for the establishment of cohesion. Both Rad30p and Eco1p need to be finely tuned in budding yeast. Given the distinct function existed in Rad30p and Eco1p, it is enigmatic how the Eso1p, the protein fusion regulated in S. pombe, works. We have identified two forms of the Eso1 protein by Western blot, and detected the Eco1-homology fragment by M/S analysis following TAP purification of Eso1 protein. The result raises the possibility that Eso1 might be processed in vivo to release the Eco1-homology fragment, which allows the independent regulation of Rad30-homology and Eco1-homology fragments.
推荐引用方式
GB/T 7714：

Chen Zhiming,Cao Hongshi,Guo Weichao, et al. Identification of two forms of the Eso1 protein in Schizosaccharomyces pombe [J].CELL BIOLOGY INTERNATIONAL,2014,38(5):682-688.
APA：

Chen Zhiming,Cao Hongshi,Guo Weichao,Lu Yingqiang.(2014).Identification of two forms of the Eso1 protein in Schizosaccharomyces pombe .CELL BIOLOGY INTERNATIONAL,38(5):682-688.
MLA：

Chen Zhiming, et al. "Identification of two forms of the Eso1 protein in Schizosaccharomyces pombe" .CELL BIOLOGY INTERNATIONAL 38,5(2014):682-688.
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