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MicroRNA-374b accelerates the development of lung cancer through downregulating PTEN expression via activating PI3K/Akt pathway 期刊论文

编号：

04f71502-aeaf-455c-85a9-1fe27f8b858c
作者：

Li, J.#^[1,5]Zhou, Z.#^[2,3]Xu, F. C.(徐凤超)^[4]Li, J.#^[1,5]Zeng, D.^[6];Cao, X. Q.^[7];Han, Y.(韩毅)*^[7]
语种：

英文
期刊：

EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES ISSN：1128-3602 2019 年 23 卷 3 期 (1116 - 1124) ; FEB
收录：
关键词：

Lung cancer MicroRNA-374b PTEN Migration Proliferation
摘要：

OBJECTIVE: To elucidate whether microRNA-374b could participate in the development of lung cancer (LC) through downregulating PTEN (gene of phosphate and tensin homolog deleted on chromosome ten) expression via activating PI3K/Akt pathway.
PATIENTS AND METHODS: Expression levels of microRNA-374b and PTEN in LC tissues and adjacent normal tissues were detected by quantitative Real Time-Polymerase Chain Reaction (qRT-PCR). Moreover, the expression level of microRNA-374b in LC cell lines was detected as well. The microRNA-374b inhibitor was constructed and transfected to downregulate microRNA-374b expression in A549 and H358 cells. The regulatory effects of microRNA-374b on migratory and proliferative capacities of LC cells were explored by wound healing and cell counting kit-8 (CCK-8) assay. respectively. After co-transfection of microRNA-374b inhibitor and si-PTEN in LC cells, expression levels of PTEN/PI3K/Akt were determined by qRT-PCR and Western blot.
RESULTS: GIRT-PCR results showed that microRNA-374b expression was higher, while PTEN expression was lower in LC tissues than adjacent tissues. Identically, microRNA-374b was also highly expressed in LC cell lines. PTEN expression was negatively correlated with microRNA-374b expression in LC. The downregulation of microRNA-374b in A549 and H358 cells inhibited their migratory and proliferative potentials. Subsequently, we verified that microRNA-374b could bind to PTEN through dual-luciferase reporter gene assay. MicroRNA-374b could inhibit PTEN expression and activate the PI3K/Akt pathway. Furthermore, PTEN knockdown enhanced migratory and proliferative abilities of LC cells. which were attenuated by co-transfection of microRNA-374b inhibitor.
CONCLUSIONS: MicroRNA-374b promotes the development of LC by downregulating PTEN expression through activating PI3K/Akt pathway.
推荐引用方式
GB/T 7714：

Li J.,Zhou Z.,Xu F. -C., et al. MicroRNA-374b accelerates the development of lung cancer through downregulating PTEN expression via activating PI3K/Akt pathway [J].EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,2019,23(3):1116-1124.
APA：

Li J.,Zhou Z.,Xu F. -C.,Li J.,&Han Y..(2019).MicroRNA-374b accelerates the development of lung cancer through downregulating PTEN expression via activating PI3K/Akt pathway .EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES,23(3):1116-1124.
MLA：

Li J., et al. "MicroRNA-374b accelerates the development of lung cancer through downregulating PTEN expression via activating PI3K/Akt pathway" .EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES 23,3(2019):1116-1124.
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