Objective: In this research, we explored the effect of long non-coding RNA (IncRNA) AOC4P on gastrointestinal stromal tumor (GIST) cells. Materials and methods: The expression of IncRNA AOC4P in tissues was detected by real-time PCR (RT-PCR). The epithelial-mesenchymal transition (EMT)-related proteins in tissues were analyzed by Western blot. The experiment included negative control group (CN), silence AOC4P group (si AOC4P), and silence negative control group (si CT). RT-PCR, MTT, Scratch, Transwell, and Annexin V-FITC methods were used to detect the expression of lncRNA AOC4P, cell proliferation, cell migration ability, cell invasion ability, and apoptosis, respectively. The EMT-related proteins including TGF-beta, ZEB1, Vimentin, Snail, and E-cadherin were analyzed by Western blot. Results: The expression of IncRNA AOC4P and the expression of EMT-related proteins in high-risk GISTS were higher than that in low- and intermediate-risk GISTS (P<0.05). It was revealed that cell proliferative migration and invasive ability in si AOC4P group was decreased than that in CN and si CT groups (P<0.05), and cell apoptosis in si AOC4P group was higher than that in si CT group. The results of Western blot demonstrated that the expression of TGF-beta 1, ZEB1, Vimentin, and Snail in si AOC4P group were lower than that in si CT and CN group (P<0.05), and the expression of E-cadherin in si AOC4P group was higher than that in si CT and CN group (P<0.05).
[1]Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Gastrointestinal Surg, Yantai, Peoples R China.
[2]Jilin Univ, Hosp 1, Dept Gastrointestinal & Anal Surg, Changchun, Jilin, Peoples R China.
[3]Qingdao Univ, Affiliated Yantai Yuhuangding Hosp, Dept Pathol, Yantai, Peoples R China.
[4]Jilin Univ, China Japan Union Union Hosp, Dept Gastrointestinal Colorectal & Anal Surg, 126 Xiantai St, Changchun 130033, Jilin, Peoples R China.
(8.0+极速模式)