Grub extract comes from Holotrichia diomphalia larvae. Numerous studies have demonstrated that grub extracts inhibit the growth of several types of cancer cells in vitro. Here, the anti-tumor activities of grub extracts were observed in experiments performed in vitro and in vivo. Human cervical carcinoma HeLa cells were treated with a range of grub extract doses. Grub extract treatment also resulted in characteristic apoptotic morphological changes in HeLa cells. In vivo acute oral toxicity tests showed that the median lethal dose (LD50) at 5 d was 48.73 g/kg (with 95% confident limits of 46.43-51.14 g/kg) in Kunming mice. Moreover, when the mice were treated with grub extracts at doses of 1.95, 3.9, and 7.8 g/(kg . d) for 10 consecutive days, the solid tumor growth of mice bearing S180 was 18.64%, 39.47%, and 53.24%, respectively, relative to the control (P< 0.05 or 0.01). These data indicate that grub extracts may inhibit tumor growth and induce apoptotic cell death, and may be a potential and promising natural product to treat tumors.
[1]Jilin Univ, Urol & Nephrol Ctr, Bethune Hosp 1, Changchun 130021, Jilin, Peoples R China.
[2]Yanbian Univ, Dept Pathol & Forens Med, Basic Med Coll, Hlth Sci Ctr, Yanbian 133000, Jilin, Peoples R China.
[3]Jilin Tianping Judicial Appraisal Ctr, Yanbian 133000, Jilin, Peoples R China.
[4]Jilin Acad Tradit Chinese Med, Dept Pathol, Changchun 130021, Jilin, Peoples R China.
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