首页 / 院系成果 / 成果详情页

Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors 期刊论文

编号：

0ad8e25a-0eee-4f19-8221-d58ee10b0c68
作者：

Sun, Dahui(孙大辉)#^[1]Junger, Wolfgang G.^[4,5];Yuan, Changji^[2];Zhang, Wenyan^[3];Bao, Yi^[4];Qin, Daming^[1];Wang, Chengxue^[1];Tan, Lei(谭磊)^[1]Qi, Baochang^[1];Zhu, Dong(朱东)^[1]Zhang, Xizheng^[6];Yu, Tiecheng(于铁成)*^[1]
语种：

英文
期刊：

STEM CELLS ISSN：1066-5099 2013 年 31 卷 6 期 (1170 - 1180) ; JUN
收录：
关键词：

Shockwaves Human mesenchymal stem cells Osteogenic differentiation ATP p38 MAPK c-Fos c-Jun
摘要：

Shockwave treatment promotes bone healing of nonunion fractures. In this study, we investigated whether this effect could be due to adenosine 5"-triphosphate (ATP) release-induced differentiation of human mesenchymal stem cells (hMSCs) into osteoprogenitor cells. Cultured bone marrow-derived hMSCs were subjected to shockwave treatment and ATP release was assessed. Osteogenic differentiation and mineralization of hMSCs were evaluated by examining alkaline phosphatase activity, osteocalcin production, and calcium nodule formation. Expression of P2X7 receptors and c-fos and c-jun mRNA was determined with real-time reverse transcription polymerase chain reaction and Western blotting. P2X7-siRNA, apyrase, P2 receptor antagonists, and p38 MAPK inhibitors were used to evaluate the roles of ATP release, P2X7 receptors, and p38 MAPK signaling in shockwave-induced osteogenic hMSCs differentiation. Shockwave treatment released significant amounts (similar to 7 mu M) of ATP from hMSCs. Shockwaves and exogenous ATP induced c-fos and c-jun mRNA transcription, p38 MAPK activation, and hMSC differentiation. Removal of ATP with apyrase, targeting of P2X7 receptors with P2X7-siRNA or selective antagonists, or blockade of p38 MAPK with SB203580 prevented osteogenic differentiation of hMSCs. Our findings indicate that shockwaves release cellular ATP that activates P2X7 receptors and downstream signaling events that caused osteogenic differentiation of hMSCs. We conclude that shockwave therapy promotes bone healing through P2X7 receptor signaling, which contributes to hMSC differentiation.
推荐引用方式
GB/T 7714：

Sun Dahui,Junger Wolfgang G.,Yuan Changji, et al. Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors [J].STEM CELLS,2013,31(6):1170-1180.
APA：

Sun Dahui,Junger Wolfgang G.,Yuan Changji,Zhang Wenyan,&Yu Tiecheng.(2013).Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors .STEM CELLS,31(6):1170-1180.
MLA：

Sun Dahui, et al. "Shockwaves Induce Osteogenic Differentiation of Human Mesenchymal Stem Cells Through ATP Release and Activation of P2X7 Receptors" .STEM CELLS 31,6(2013):1170-1180.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：49  下载次数：0

分享到：

浏览次数：49

下载次数：0

打印次数：0