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Hepatitis C virus core protein triggers expansion and activation of CD4(+) CD25(+) regulatory T cells in chronic hepatitis C patients 期刊论文

编号：

0ecf0ef6-02b4-47c4-8b35-696c3cec92ef
作者：

Zhai, Naicui(翟乃翠)#^[1]Chi, Xiumei(迟秀梅)^[2]Li, Tianyang^[1];Song, Hongxiao^[1];Li, Haijun(李海军)^[1]Jin, Xia^[3];Crispe, Ian Nicholas^[4];Su, Lishan^[1,5];Niu, Junqi(牛俊奇)^[2]Tu, Zhengkun(涂正坤)*^[1,2]
语种：

英文
期刊：

CELLULAR & MOLECULAR IMMUNOLOGY ISSN：1672-7681 2015 年 12 卷 6 期 (743 - 749) ; NOV
收录：
关键词：

CD4(+) CD25(+) regulatory T cells HCV core Toll-like receptor
摘要：

CD4(+) CD25(+) FoxP3(+) regulatory T cells (Tregs) are increased in patients with chronic hepatitis C, which may contribute to the sustained suppression of hepatitis C virus (HCV)-specific T-cell responses and viral persistence in HCV-infected individuals. We postulated that HCV core protein (HCVc) directly contributes to the expansion of Tregs in HCV-infected patients, and we provide evidence to support this hypothesis in the report. Peripheral blood mononuclear cells (PBMCs) and sera were collected from 87 treatment-naive chronic HCV-infected patients, CD4(+) CD25(+) Tregs were measured by flow cytometry, and HCV RNA and HCVc levels were detected using qPCR and enzyme-linked immunosorbent assay (ELISA), respectively. CD4(+), CD8(+), CD4(+) CD25(+) and CD4(+) CD25(-) T cells were purified from healthy donors and cultured with recombinant HCVc and Toll-like receptor (TLR) ligands. Flow cytometry was used to analyze cell proliferation, and ELISA was performed to measure cytokine production. In the 87 chronic HCV-infected patients, HCVc showed a significant correlation with HCV RNA and CD4(+) CD25(+) Tregs. Mechanistic studies showed that HCVc, together with anti-CD3 antibody, augmented CD4(+) CD25(+) Treg proliferation, but inhibited CD4(+) CD25(-) T-cell proliferation and IFN-gamma production, in a dose-dependent and Treg-dependent manner. Moreover, unlike the TLR3 ligand (poly I: C) and the TLR4 ligand (lipopolysaccharide, LPS), the TLR2 ligand (lipoteichoic acid, LTA) and HCVc both inhibited TCR-induced CD4(+) T-cell proliferation and IFN-gamma secretion in a Treg-dependent manner. These data indicate that HCVc, like other TLR2 ligands, triggers CD4(+) CD25(+) Treg activation and expansion to inhibit host immune responses, which may play a critical role in viral persistence in HCV-infected patients.
推荐引用方式
GB/T 7714：

Zhai Naicui,Chi Xiumei,Li Tianyang, et al. Hepatitis C virus core protein triggers expansion and activation of CD4(+) CD25(+) regulatory T cells in chronic hepatitis C patients [J].CELLULAR & MOLECULAR IMMUNOLOGY,2015,12(6):743-749.
APA：

Zhai Naicui,Chi Xiumei,Li Tianyang,Song Hongxiao,&Tu Zhengkun.(2015).Hepatitis C virus core protein triggers expansion and activation of CD4(+) CD25(+) regulatory T cells in chronic hepatitis C patients .CELLULAR & MOLECULAR IMMUNOLOGY,12(6):743-749.
MLA：

Zhai Naicui, et al. "Hepatitis C virus core protein triggers expansion and activation of CD4(+) CD25(+) regulatory T cells in chronic hepatitis C patients" .CELLULAR & MOLECULAR IMMUNOLOGY 12,6(2015):743-749.
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