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DNA damage induced by oridonin involves cell cycle arrest at G2/M phase in human MCF-7 cells 期刊论文

编号：

10af6a35-1bba-48a4-b2f7-d1872f594c5c
作者：

Zhang, Tao(张涛)#^[1,2]Tan, Yan*^[1,3]Zhao, Rui^[4];Liu, Zhaoyang^[5];
语种：

英文
期刊：

WSPOLCZESNA ONKOLOGIA-CONTEMPORARY ONCOLOGY ISSN：1428-2526 2013 年 17 卷 1 期 (38 - 44) ; FEB
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关键词：

oridonin ATM CHK2 cell cycle p53 apoptosis
摘要：

Aim of the study: To study the Mechanisms of inhibition of tell growth and induction of DNA damage in oridonin-treated MCF-7 human breast cancer cells.
Material and Methods: The cytotoxicity of oridonin-treated MCF-7 cells was measured by MTT assay. Cell cycle phase distribution was analyzed by flow cytometry. P-ATM, P-CHK2, gamma H2AX and P-P53 protein expressions were detected by Western blot analysis. The expression of r-h2ax and P-ATM was also detected by immunofluorescence staining. The degree of cellular damage of oridonin-induced MCF-7 human breast cancer cells was confirmed by the comet assay analysis of DNA fragmentation.
Results: Oridonin inhibited cell growth in a time- and dose-dependent manner. The IC50 values at 48 and 72 hours were 783 and 31.62 mu mol/l, respectively. Oridonin induced G2/M phase arrest in MCF-7 tells. MCF-7 cells treated with oridonin showed significant DNA damage as shown by an increase in olive tail moment (OTM). The protein expression levels of P-ATM, P-CHK2, gamma H2AX and P-P53 were increased significantly in a dose-dependent manner.
Conclusions: DNA damage provokes p53-mediated G2/M tell cycle arrest in oridonin-induced MCF-7 cells through the mechanism of CHK2 activation by activated ATM protein kinase, which is induced by oridonin.
推荐引用方式
GB/T 7714：

Zhang Tao,Tan Yan,Zhao Rui, et al. DNA damage induced by oridonin involves cell cycle arrest at G2/M phase in human MCF-7 cells [J].WSPOLCZESNA ONKOLOGIA-CONTEMPORARY ONCOLOGY,2013,17(1):38-44.
APA：

Zhang Tao,Tan Yan,Zhao Rui,Liu Zhaoyang.(2013).DNA damage induced by oridonin involves cell cycle arrest at G2/M phase in human MCF-7 cells .WSPOLCZESNA ONKOLOGIA-CONTEMPORARY ONCOLOGY,17(1):38-44.
MLA：

Zhang Tao, et al. "DNA damage induced by oridonin involves cell cycle arrest at G2/M phase in human MCF-7 cells" .WSPOLCZESNA ONKOLOGIA-CONTEMPORARY ONCOLOGY 17,1(2013):38-44.
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