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IL-beta Induces IL-6 production in retinal Muller cells predominantly through the activation of P38 MAPK/NF-kappa B signaling pathway 期刊论文

编号：

1e81b415-42c5-4739-a57e-070107df3d56
作者：

Liu, Xiufen(刘秀芬)#^[1]Ye, Fei^[2];Xiong, Huabao^[3];Hu, DanNing^[4];Limb, G. Astrid^[5];Xie, Tian^[6];Peng, Liang^[3];Zhang, Pili^[7];Wei, Yi^[8];Zhang, Wiley^[2];Wang, Juan^[3];Wu, Hongwei^[2];Lee, Peng^[9,10];Song, E.(宋鄂)*^[1,11]Zhang, David Y.*^[2]
语种：

英文
期刊：

EXPERIMENTAL CELL RESEARCH ISSN：0014-4827 2015 年 331 卷 1 期 (223 - 231) ; FEB 1
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关键词：

Muller cells Interleukin 1 beta (IL-1 beta) Interleukin 6 (IL-6) p38 MAPK NF-kappa B Promoter
摘要：

IL-6 plays an important role in various inflammatory ocular diseases, including diabetic retinopathy. Muller cells are the major source of inflammatory mediators, including IL-6, in the retina. However, the mechanism of regulating IL-6 production in these cells remains unclear. Examination of signaling pathways in human retinal Muller cells (MID-MI cell line) cultured with IL-10, TNF-a, 1-6, IL-8, VEGF, IFN-y, glucose or mannitol showed that IL-1f3 was the most potent stimulator of IL-6 production. In addition, IL-1 p also increased NF-x13 p50 protein level and phosphorylation of p38 MAPK, ERK1/2 and c-Jun. Induction of IL-6 production by IL-10 was significantly reduced by addition of p38 MAPK (SB203580), MEK1/2 (U0126) or NF-0 (BAY117082) inhibitors, with the highest effect being observed with SB203580. To explore the specific elements in IL-6 promoter responsible for IL-l3-induction of IL-6 expression, a series of plasmids bearing various IL-6 promoter mutations were transiently expressed in MID-MI cells cultured in the presence or absence of IL-lp (10 ng/ml) and/or SB203580 (1011M). Results showed that IL-6 promoter activity of the parent pIL-6-Luc651 was significantly enhanced by IL-l13, but the level was significantly attenuated by SB203580. Furthermore, the IL-6 promoter activity was also reduced upon deletion of NF-1(13, AP-1 or C/EBP binding sites, with NF-KB deletion being the greatest. These results are the first demonstration that IL-l1 induces IL-6 production in Muller cells by activation of IL-6 promoter activity predominantly through the p38 MAPK/NF-KB pathway. (C) 2014 Elsevier Inc. All rights reserved.
推荐引用方式
GB/T 7714：

Liu Xiufen,Ye Fei,Xiong Huabao, et al. IL-beta Induces IL-6 production in retinal Muller cells predominantly through the activation of P38 MAPK/NF-kappa B signaling pathway [J].EXPERIMENTAL CELL RESEARCH,2015,331(1):223-231.
APA：

Liu Xiufen,Ye Fei,Xiong Huabao,Hu Dan-Ning,&Zhang David Y..(2015).IL-beta Induces IL-6 production in retinal Muller cells predominantly through the activation of P38 MAPK/NF-kappa B signaling pathway .EXPERIMENTAL CELL RESEARCH,331(1):223-231.
MLA：

Liu Xiufen, et al. "IL-beta Induces IL-6 production in retinal Muller cells predominantly through the activation of P38 MAPK/NF-kappa B signaling pathway" .EXPERIMENTAL CELL RESEARCH 331,1(2015):223-231.
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