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Inhibition of cell proliferation through an ATP-responsive co-delivery system of doxorubicin and Bcl-2 siRNA 期刊论文

编号：

221c67dd-2564-4f22-8db1-47cddad0517e
作者：

Zhang, Jianxu#^[1,2]Wang, Yudi#^[2]Chen, Jiawen^[2];Liang, Xiao^[2];Han, Haobo^[1,2];Yang, Yan^[2];Li, Quanshun(李全顺)*^[1,2]Wang, Yanbo(王艳波)*^[1]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF NANOMEDICINE ISSN：1178-2013 2017 年 12 卷 (4721 - 4732) ; 2017
收录：
关键词：

ATP response aptamer doxorubicin Bcl-2 siRNA anti-proliferation synergistic effect
摘要：

Herein, DNA duplex was constructed through the hybridization of adenosine triphosphate (ATP)-responsive aptamer and its cDNA in which GC-rich motif could be used to load doxorubicin (DOX), and then, cationic polymer PEI25K was used as a carrier to simultaneously condense DOX-Duplex and Bcl-2 siRNA to prepare the ternary nanocomplex polyethylenimine (PEI)/DOX-Duplex/siRNA. The ATP concentration gradient between the cytosol and extracellular environment could achieve the stable loading of DOX in duplex and the rapid drug release in an ATP-responsive manner. Using human prostate tumor cell line PC-3 as a model, an obvious induction of cell proliferation could be detected with a cell viability of 53.3%, which was stronger than single cargo delivery, indicating the synergistic effect between these two components. The enhanced anti-proliferative effect of ternary nanocomplex could be attributed to the improved induction of cell apoptosis in a mitochondria-mediated pathway and cell-cycle arrest at the G2 phase. Overall, the ATP-responsive nanocarrier for co-delivering DOX and Bcl-2 siRNA has been demonstrated to be a smart delivery system with favorable anti-proliferative effect, especially for solving the multidrug resistance of tumors.
推荐引用方式
GB/T 7714：

Zhang Jianxu,Wang Yudi,Chen Jiawen, et al. Inhibition of cell proliferation through an ATP-responsive co-delivery system of doxorubicin and Bcl-2 siRNA [J].INTERNATIONAL JOURNAL OF NANOMEDICINE,2017,12:4721-4732.
APA：

Zhang Jianxu,Wang Yudi,Chen Jiawen,Liang Xiao,&Wang Yanbo.(2017).Inhibition of cell proliferation through an ATP-responsive co-delivery system of doxorubicin and Bcl-2 siRNA .INTERNATIONAL JOURNAL OF NANOMEDICINE,12:4721-4732.
MLA：

Zhang Jianxu, et al. "Inhibition of cell proliferation through an ATP-responsive co-delivery system of doxorubicin and Bcl-2 siRNA" .INTERNATIONAL JOURNAL OF NANOMEDICINE 12(2017):4721-4732.
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