The characteristics of type II microcalcifications in fibroadenoma (FB), atypical ductal hyperplasia (ADH), and ductal carcinoma in situ (DCIS) breast tissues has been analyzed by the fingerprint features of Raman spectroscopy. Fresh breast tissues were first handled to frozen sections and then they were measured by normal Raman spectroscopy. Due to inherently low sensitivity of Raman scattering, Au@SiO2 shell-isolated nanoparticle-enhanced Raman spectroscopy (SHINERS) technique was utilized. A total number of 71 Raman spectra and 70 SHINERS spectra were obtained from the microcalcifications in benign and premalignant breast tissues. Principal component analysis (PCA) was used to distinguish the type II microcalcifications between these tissues. This is the first time to detect type II microcalcifications in premalignant (ADH and DCIS) breast tissue frozen sections, and also the first time SHINERS has been utilized for breast cancer detection. Conclusions demonstrated in this paper confirm that SHINERS has great potentials to be applied to the identification of breast lesions as an auxiliary method to mammography in the early diagnosis of breast cancer. (C) 2014 Elsevier B.V. All rights reserved.
[1]Jilin Univ, State Key Lab Supramol Struct & Mat, Changchun 130021, Peoples R China.
[2]Jilin Univ, Hosp 1, Dept Breast Surg, Changchun 130021, Peoples R China.
[3]Jilin Univ, China Japan Union Hosp, Dept Radiotherapy, Changchun 130021, Peoples R China.
[4]Jilin Univ, Hosp 1, Dept Pathol, Changchun 130021, Peoples R China.
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