MicroRNAs are emerging as critical regulators in cerebral ischemia/reperfusion injury; however, their exact roles remain poorly understood. miR-153 is reported to be a neuron-related miRNA involved in neuroprotection. In this study, we aimed to investigate the precise role of miR-153 in regulating neuron survival during cerebral ischemia/reperfusion injury using an oxygen-glucose deprivation and reoxygenation (OGD/R) cellular model. We found that miR-153 was significantly upregulated in neurons subjected to OGD/R treatment. Inhibition of miR-153 significantly attenuated OGD/R-induced injury and oxidative stress in neurons. Nuclear factor erythroid 2-related factor 2 (Nrf2) was identified as a target gene of miR-153. Inhibition of miR-153 significantly promoted the expression of Nrf2 and heme oxygenase-1 (HO-1). However, silencing of Nrf2 significantly blocked the protective effects of miR-153 inhibition. Our study indicates that the inhibition of miR-153 protects neurons against OGD/R-induced injury by regulating Nrf2/HO-1 signaling and suggests a potential therapeutic target for cerebral ischemia/reperfusion injury.
[1]Jilin Univ, Dept Pharmacol, Coll Basic Med Sci, Changchun 130021, Jilin, Peoples R China.
[2]Jilin Univ, Dept Neonatol, Hosp 1, Changchun 130021, Jilin, Peoples R China.
[3]Jilin Univ, Dept Pediat Neurol, Hosp 1, Changchun 130021, Jilin, Peoples R China.
[4]Jilin Univ, Hosp 1, Cadres Ward, Changchun 130021, Jilin, Peoples R China.
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