Gastric cancer remains the fourth most commonly diagnosed cancer and is the second leading cause of cancer-related mortality worldwide. The aim of this study was to investigate the effects of canolol on the proliferation and apoptosis of SGC-7901 human gastric cancer cells. and its relevant molecular mechanisms. The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to observe the effect of canolol on the proliferation of SGC-7901 human gastric adenocarcinoma cells. The results showed that SGC-7901 cells exhibited a marked dose-dependent reduction in the proliferation rate. The survival rate of the cells was 88.86 +/- 1.58% at 50 mu mol/l, decreasing to 53.73 +/- 1.51% at 800 mu mol/l (P<0.05). By contrast, canolol had no significant toxicity on the human gastric mucosal epithelial cell line GES-1. The vivid images of cell morphology using an inverted microscope provided confirmation of the MTT assay. Treatment of SGC-7901 cells with canolol resulted in apoptosis demonstrated by flow cytometry. Furthermore, canolol downregulated the mRNA levels of COX-2, but had no significant effect on the mRNA expession of the Bax and Bcl-2 genes. These findings suggest that canolol has potential to be developed as a new natural anti-gastric carcinoma agent.
[1]Jilin Univ, Hosp 1, Div Clin Epidemiol, Changchun 130021, Jilin, Peoples R China.
[2]Fujita Hlth Univ, Sch Med, Div Pathol, Toyoake, Aichi 47011, Japan.
[3]Jilin Univ, Norman Bethune Med Coll, Dept Pathogeny Biol, Changchun 130021, Jilin, Peoples R China.
[4]Jilin Univ, Hosp 1, Dept Gastr & Colorectal Surg, Changchun 130021, Jilin, Peoples R China.
(8.0+极速模式)