OBJECTIVE: Cervical squamous cell carcinoma seriously threats to patient"s life and health. MiRNAs have role of regulating cell growth, proliferation, and death. MiRNAs can promote or inhibit cell growth and proliferation. This study discussed the role of miRNA373 in regulating cervical squamous cell carcinoma growth, proliferation, and apoptosis.
PATIENTS AND METHODS: MiRNA373 and scramble miRNA were synthetized and transfected to cervical squamous cell carcinoma SiHa cells by lipofectamine. IAPs plasmid and miRNA373 were sequentially transfected to SiHa cells. MTT assay, caspase-3 activity, and flow cytometry were applied to test miRNA373 and IAPs impacts on cell growth, proliferation, and apoptosis. Western blot was adopted to determine IAPs expression.
RESULTS: MiRNA373 transfection obviously reduced SiHa cell growth, induced phosphatidylserine eversion and caspase-3 activation, and declined IAPs expression. IAPs interference significantly enhanced miRNA373 induced SiHa cell apoptosis. IAPs overexpression markedly restrained miRNA373 induced SiHa cell apoptosis.
CONCLUSIONS: MiRNA373 transfection suppressed SiHa cell growth and proliferation. MiRNA373 induced SiHa cell apoptosis possibly through downregulating IAPs, suggesting that IAPs might be a target for cervical squamous cell carcinoma treatment.
[1]Jilin Univ, Dept Gynaecol & Obstet, Hosp 2, 218 Ziqiang St, Changchun 130041, Jilin, Peoples R China.
[2]Jilin Univ, Bethune Sch Med, Changchun 130021, Jilin, Peoples R China.
[3]Jilin Univ, Bethune Med Coll, Dept Pathol & Pathophysiol, Changchun 130021, Jilin, Peoples R China.
[4]Jilin Univ, Dept Pediat Surg, Hosp 1, Changchun 130021, Jilin, Peoples R China.
(8.0+极速模式)