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IL-4-polarized BV2 microglia cells promote angiogenesis by secreting exosomes 期刊论文

编号：

37e8a8c5-99e9-4fc2-90e2-277ca06e37c7
作者：

Tian, Yuan#^[1,2]Zhu, Pei^[1];Liu, Shanshan^[1];Lin, Zheng^[1];Li, Dong^[1];Zhao, Heng^[3];Zhu, Xun^[1];Shu, Chang(舒畅)^[4]Yan, Dongmei*^[1]Dong, Zehua^[5];
语种：

英文
期刊：

ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE ISSN：1899-5276 2019 年 28 卷 4 期 (421 - 429) ; APR
收录：
关键词：

angiogenesis exosomes microglia interleukin 4
摘要：

Background. The microglia cell transfer has been shown to play a protective role in ischemic stroke. Microglia cells may play this nerve-protective role via the promotion of angiogenesis. However, the underlying mechanisms are largely unknown and need further investigation.
Objectives. The aim of this study was to investigate the pro-angiogenesis effects of unpolarized, interleukin 4 (IL-4)-polarized or lipopolysaccharide (LPS)-polarized BV2 microglia cells both in vivo and in vitro. We also investigated the potential mechanisms of these pro-angiogenesis effects.
Material and methods. BV2 cells were polarized using phosphate-buffered saline (PBS), LPS or IL-4, respectively. The gene expression pattern was analyzed with reverse transcription polymerase chain reaction (RT-PCR). The transfer of polarized BV2 cells was performed with an intravenous injection into mice 45 min after the middle cerebral artery (MCA) occlusion. Angiogenin expression was assessed with immunofluorescence. We also examined the angiogenesis effect of polarized BV2 cells and their exosomes through 3-dimensional co-cultures in vitro. Finally, the microRNA (miRNA) profiles of exosomes released by BV2 cells under different polarization conditions were examined using miRNA microarray.
Results. The IL-4-polarized BV2 transplantation promoted angiogenin expression in the ischemic brain. Interleukin 4-polarized microglia increased the tube formation of endothelial cells by secreting exosomes. The miRNA profiles of exosomes released by BV2 cells under different polarization conditions were different. Exosomes from IL-4-polarized BV2 cells contained higher amounts of miRNA-26a compared to those from the LPS-polarized and unpolarized BV2 cells.
Conclusions. Interleukin 4-polarized microglia cells might ameliorate the damage caused by ischemic stroke by promoting angiogenesis through the secretion of exosomes containing miRNA-26a.
推荐引用方式
GB/T 7714：

Tian Yuan,Zhu Pei,Liu Shanshan, et al. IL-4-polarized BV2 microglia cells promote angiogenesis by secreting exosomes [J].ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE,2019,28(4):421-429.
APA：

Tian Yuan,Zhu Pei,Liu Shanshan,Lin Zheng,&Dong Zehua.(2019).IL-4-polarized BV2 microglia cells promote angiogenesis by secreting exosomes .ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE,28(4):421-429.
MLA：

Tian Yuan, et al. "IL-4-polarized BV2 microglia cells promote angiogenesis by secreting exosomes" .ADVANCES IN CLINICAL AND EXPERIMENTAL MEDICINE 28,4(2019):421-429.
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