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Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells 期刊论文

编号：

3bf9e142-1cc7-4793-8ba4-0b7970e94391
作者：

Yu Haipeng(于海鹏)#^[2]Xue Yan^[2];An Wei(安伟)^[4]Liu Dan^[1];Hao Shumei^[3];Sheng Jun(盛军)*^[1]
语种：

英文
期刊：

CHEMICAL RESEARCH IN CHINESE UNIVERSITIES ISSN：1005-9040 2009 年 25 卷 5 期 (695 - 698) ; SEP 2
收录：
关键词：

Transgenic cell HBsAg Ginseng
摘要：

The recombinant plasmid pBIBSa containing the HBsAg DNA fragment was transferred into Agrobacterium tumefaciens strain LBA4404 directly. Ginseng cells were transfected with A. tumefaciens carrying pBIBSa and the ginseng cell lines carrying HBsAg-S gene were obtained. The presence of target gene in the transfect cells was confirmed by PCR and RT-PCR. A clear band at the site of 700 bp was observed by agarose electrophoresis analysis of the samples containing the target gene. HBsAg expressed by the transgenic ginseng cells was detected by Western blot. Maximum expression levels of 184 ng HBsAg/g FW and 0.009% of the total soluble proteins were observed by ELISA. HBsAg in ginseng cells was located both on the cell membrane and in the nuclei.
推荐引用方式
GB/T 7714：

Yu Hai-peng,Xue Yan,An Wei, et al. Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells [J].CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,2009,25(5):695-698.
APA：

Yu Hai-peng,Xue Yan,An Wei,Liu Dan,&Sheng Jun.(2009).Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells .CHEMICAL RESEARCH IN CHINESE UNIVERSITIES,25(5):695-698.
MLA：

Yu Hai-peng, et al. "Expression of Hepatitis B Surface Antigen Gene in Ginseng Cells" .CHEMICAL RESEARCH IN CHINESE UNIVERSITIES 25,5(2009):695-698.

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