Osteoblasts dysfunction, induced by aluminum (Al), plays a critical role in the osteoporosis etiology. Ginsenoside Rb1 (Rb1) has the therapeutic properties for osteoporosis. This study aimed to assess the efficiency of Rb1 in ameliorating Al-induced osteoblasts dysfunction. The osteoblasts were divided into four groups: Rb1-treated group (RG, 0.0145 mg/mL Rb1), control group (CG, 0), AlCl3-treated group (AG, 0.126 mg/mL AlCl3 center dot 6H(2)O), AlCl3 + Rb1-treated group (ARG, 0.0145 mg/mL Rb1 and 0.126 mg/mL AlCl3 center dot 6H(2)O). After 24 h of culture, the osteoblasts viability, the transforming growth factor-beta 1 (TGF-(beta 1)), bone morphogenetic protein-2 (BMP-2), the insulin-like growth factor I (IGF-I), core-binding factor alpha 1 (Cbf alpha 1) mRNA expressions, glutathione perioxidase (GSH-P-x) and superoxide dismutase (SOD) activities, and reactive oxygen species (ROS) concentration were determined. The osteoblasts ultrastructural features were also observed. In the ARG, the osteoblasts viability, TGF-(beta 1), BMP-2, IGF-I and Cbf alpha 1 mRNA expressions and the GSH-P-X and SOD activities were significantly increased, the ROS concentration was significantly decreased, and osteoblasts histology lesion was attenuated compared with the AG. These results demonstrated that Rb1 could significantly reverse osteoblasts viability and osteoblasts growth regulation factor, inhibit oxidative stress, and attenuate histology lesion in the osteoblasts with AlCl3. These results indicate that Rb1 can effectively alleviate the AlCl3-induced osteoblasts dysfunction. (C) 2016 Elsevier Ireland Ltd. All rights reserved.
[1]Chinese Acad Agr Sci, Inst Special Anim & Plant Sci, Changchun 130112, Peoples R China.
[2]Fujian Agr & Forestry Univ, Coll Anim Sci, Fuzhou 350002, Peoples R China.
[3]Jilin Univ, Hosp 1, Dept Vasc Surg, Changchun 130021, Peoples R China.
[4]Northeast Agr Univ, Coll Vet Med, Harbin 150030, Peoples R China.
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