Methyl jasmonate potentially induces the differentiation of human myeloid leukemia cells and inhibits their proliferation; it may induce the differentiation and apoptosis of human lymphocytic leukemia cells, but does not exert a damaging effect on normal lymphocytes. In the present study, the anticancer effect of methyl jasmonate on human colorectal cancer cells was investigated. Cell viability and apoptosis was assessed using a Cell Counting kit-8 assay and flow cytometry, respectively. Methyl jasmonate suppressed cell viability and induced apoptosis in human colorectal cancer cells. Additionally, methyl jasmonate increased the activation of caspase-3, inhibited the expression levels of enhancer of zeste 2 polycomb repressive complex 2 subunit (EZH2) and the Wnt beta-catenin pathway in human colorectal cancer. Downregulation of EZH2 expression enhanced the anticancer effect of methyl jasmonate on human colorectal cancer cells through suppression of the Wnt beta-catenin pathway. Thus, EZH2 downregulation promotes the anticancer effect of methyl jasmonate by inducing apoptosis in human colorectal cancer cells through the Wnt/beta-catenin pathway.
[1]Jilin Univ, China Japan Union Hosp, Dept Urol, Changchun 1300334, Jilin, Peoples R China.
[2]Jilin Univ, China Japan Union Hosp, Dept Galactophore, Changchun 1300334, Jilin, Peoples R China.
[3]Jilin Univ, China Japan Union Hosp, Dept Orthoped, Changchun 1300334, Jilin, Peoples R China.
[4]Jilin Univ, Hosp 1, Canc Ctr, 71 Xinmin St, Changchun 130021, Jilin, Peoples R China.
(8.0+极速模式)