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Glucose deprivation promotes apoptotic response to S1 by enhancing autophagy in human cervical cancer cells 期刊论文

编号：

4da30598-0eac-4e79-b0d0-60bb6552e0e4
作者：

Zhouu, Li#^[1,2]Kong, Qinghuan^[3];Zhang, Yunhan^[3];Yang, Wei(杨威)^[4]Yan, Shan^[3];Li, Meihui^[3];Wang, Yidan^[3];Zhou, Yanjie^[3];Yu, Huimei*^[3]Han, Liying(韩丽英)*^[1]
语种：

English
期刊：

CANCER MANAGEMENT AND RESEARCH ISSN：1179-1322 2018 年 10 卷 (6195 - 6204)
收录：
关键词：

Earle's balanced salt solution glucose deprivation S1 endoplasmic reticulum stress autophagy apoptosis
摘要：

Background: S1 is a novel BH3 mimetic that can induce death in some types of cancer cells, such as melanoma B16, ovarian cancer SKOV3, and U251 glioma cells. S1 inhibits Bcl-2 and Mcl-1 expression and induces cancer cell apoptosis. Cancer cell survival is highly dependent on glucose. Here, we observed the effect of glucose deprivation on the apoptotic response to S1 in human cervical cancer (HeLa) cells. Materials and methods: Earle's balanced salt solution (EBSS) was used to simulate glucose deprivation. MTT assay was used to analyze the cell survival rate, and Hoechst 33342 dye was used to detect the apoptosis in HeLa cells. Western blotting was used to detect the expression of ER stress and autophagy relative proteins. In addition, lysosomes were observed with Lyso-Tracker staining by confocal microscopy. Results: S1 decreased cell distribution density and survival rate, and MTT assay showed that EBSS enhanced the inhibitory effects of S1 on HeLa cell growth. Hoechst 33342 dye showed that S1 led to pyknosis, fragmentation, and strong staining in HeLa cell nuclei, and EBSS enhanced these effects. Western blotting indicated that EBSS enhanced the expression of apoptosis-related proteins (cytochrome C, caspase-3, and poly[ADP-ribose] polymerase 1) induced by S1 in HeLa cells. S1 decreased p62 expression and increased the autophagosome-associated protein LC3-II expression, which indicated that S1 induced autophagy in HeLa cells. EBSS enhanced S1-induced autophagy in HeLa cells. Furthermore, autophagy inhibitor chloroquine enhanced S1-induced apoptosis in HeLa cells. Conclusion: These results indicate that EBSS exacerbates S1-induced autophagy and severe autophagy induced by EBSS and SI could lead to apoptosis in HeLa cells. The results also suggest that EBSS enhances the sensitivity of HeLa cells to S1-induced apoptosis and that autophagy plays an important role in this process.
推荐引用方式
GB/T 7714：

Zhouu Li,Kong Qinghuan,Zhang Yunhan, et al. Glucose deprivation promotes apoptotic response to S1 by enhancing autophagy in human cervical cancer cells [J].CANCER MANAGEMENT AND RESEARCH,2018,10:6195-6204.
APA：

Zhouu Li,Kong Qinghuan,Zhang Yunhan,Yang Wei,&Han Liying.(2018).Glucose deprivation promotes apoptotic response to S1 by enhancing autophagy in human cervical cancer cells .CANCER MANAGEMENT AND RESEARCH,10:6195-6204.
MLA：

Zhouu Li, et al. "Glucose deprivation promotes apoptotic response to S1 by enhancing autophagy in human cervical cancer cells" .CANCER MANAGEMENT AND RESEARCH 10(2018):6195-6204.
入库时间：

5/20/2019 5:32:42 PM
更新时间：

5/20/2019 5:32:42 PM
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