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Recombinant Human Erythropoietin Protects Myocardial Cells from Apoptosis via the Janus-Activated Kinase 2/Signal Transducer and Activator of Transcription 5 Pathway in Rats with Epilepsy 期刊论文

编号：

59c0f5cd-5f44-40ec-9019-cc017a53ed9e
作者：

Ma, BaoXin#^[1]Li, Jie(李杰)#^[2]Li, Hua#^[1]Wu, SuiSheng(吴绥生)*^[2]
语种：

英文
期刊：

CURRENT THERAPEUTIC RESEARCH-CLINICAL AND EXPERIMENTAL ISSN：0011-393X 2015 年 77 卷 (90 - 98) ; DEC
收录：
关键词：

apoptosis epilepsy erythropoietin JAK2 STAT5
摘要：

Objective: To investigate the potential mechanisms underlying the protective effects of recombinant human erythropoietin (rhEPO) and carbamylated EPO (CEPO) against myocardial cell apoptosis in epilepsy.
Methods: Rats were given an intra-amygdala injection of kainic acid to induce epilepsy. Groups of rats were treated with rhEPO or CEPO before induction of epilepsy, whereas additional rats were given a caudal vein injection of AG490, a selective inhibitor of Janus kinase 2 (JAK2). At different time points after seizure onset, electroencephalogram changes were recorded, and myocardium samples were taken for the detection of myocardial cell apoptosis and expression of JAK2, signal transducer and activator of transcription 5 (STAT5), caspase-3, and bcl-xl mRNA5 and proteins.
Results: Induction of epilepsy significantly enhanced myocardial cell apoptosis and upregulated the expression of caspase-3 and bcl-xl proteins and JAK2 and STAT5a at both the mRNA and protein levels. Pretreatment with either rhEPO or CEPO reduced the number of apoptotic cells, upregulated bcl-xl expression, and downregulated caspase-3 expression in the myocardium of epileptic rats. Both myocardial JAK2 and STAT5a mRNA5, as well as phosphorylated species of JAK2 and STAT5a, were upregulated in epileptic rats in response to rhEPO but not to CEPO pretreatment. AG490 treatment increased apoptosis, upregulated caspase-3 protein expression, and downregulated bcl-xl protein expression in the myocardium of epileptic rats.
Conclusions: These results indicate that myocardial cell apoptosis may contribute to myocardial injury in epilepsy. EPO protects myocardial cells from apoptosis via the JAK2/STAT5 pathway in rats with experimental epilepsy, whereas CEPO exerts antiapoptotic activity perhaps via a pathway independent of JAK2/STAT5 signaling. (C)2015. The Authors. Published by Elsevier Inc. This is an open access article under the CC BY-NC-ND license.
推荐引用方式
GB/T 7714：

Ma Bao-Xin,Li Jie,Li Hua, et al. Recombinant Human Erythropoietin Protects Myocardial Cells from Apoptosis via the Janus-Activated Kinase 2/Signal Transducer and Activator of Transcription 5 Pathway in Rats with Epilepsy [J].CURRENT THERAPEUTIC RESEARCH-CLINICAL AND EXPERIMENTAL,2015,77:90-98.
APA：

Ma Bao-Xin,Li Jie,Li Hua,Wu Sui-Sheng.(2015).Recombinant Human Erythropoietin Protects Myocardial Cells from Apoptosis via the Janus-Activated Kinase 2/Signal Transducer and Activator of Transcription 5 Pathway in Rats with Epilepsy .CURRENT THERAPEUTIC RESEARCH-CLINICAL AND EXPERIMENTAL,77:90-98.
MLA：

Ma Bao-Xin, et al. "Recombinant Human Erythropoietin Protects Myocardial Cells from Apoptosis via the Janus-Activated Kinase 2/Signal Transducer and Activator of Transcription 5 Pathway in Rats with Epilepsy" .CURRENT THERAPEUTIC RESEARCH-CLINICAL AND EXPERIMENTAL 77(2015):90-98.
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