Repetitive exposure of diabetic mice to low-dose radiation (LDR) at 25 mGy could significantly attenuate diabetes-induced renal inflammation, oxidative damage, remodeling, and dysfunction, for which, however, the underlying mechanism remained unknown. The present study explored the effects of LDR on the expression and function of Akt and Nrf2 in the kidney of diabetic mice. C57BL/6J mice were used to induce type 1 diabetes with multiple low-dose streptozotocin. Diabetic and age-matched control mice were irradiated with whole body X-rays at either single 25 mGy and 75 mGy or accumulated 75 mGy (25 mGy daily for 3 days) and then sacrificed at 1-12 h for examining renal Akt phosphorylation and Nrf2 expression and function. We found that 75 mGy of X-rays can stimulate Akt signaling pathway and upregulate Nrf2 expression and function in diabetic kidneys; single exposure of 25 mGy did not, but three exposures to 25 mGy of X-rays could offer a similar effect as single exposure to 75 mGy on the stimulation of Akt phosphorylation and the upregulation of Nrf2 expression and transcription function. These results suggest that single 75 mGy or multiple 25 mGy of X-rays can stimulate Akt phosphorylation and upregulate Nrf2 expression and function, which may explain the prevention of LDR against the diabetic nephropathy mentioned above.
[1]Jilin Univ, Sch Publ Hlth, Changchun 130021, Peoples R China.
[2]Wenzhou Med Coll, Chinese Amer Res Inst Diabet Complicat, Wenzhou 325035, Peoples R China.
[3]Univ Louisville, Sch Med, Dept Pediat, Louisville, KY 40059 USA.
[4]Jilin Univ, Norman Bethune Coll Med, Changchun 130021, Peoples R China.
[5]Jilin Univ, Norman Bethune Hosp 1, Changchun 130021, Peoples R China.
[6]Jilin Agr Univ, Engn Res Ctr Bioreactor & Pharmaceut Dev, Changchun 130118, Peoples R China.
[7]Univ Louisville, Sch Med, Dept Pharmacol & Toxicol, Louisville, KY 40059 USA.
[8]Univ Louisville, Sch Med, Dept Radiat Oncol, Louisville, KY 40059 USA.
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