This study measured the levels of expression of CD133, hypoxia-inducible factor (HIF)-1 alpha and vascular endothelial growth factor (VEGF) in human prostate cancer cells grown under hypoxic and non-hypoxic conditions to compare the values to resulting amounts of proliferation and apoptosis in the cells. Human prostate cancer cell line LNCaP cells were routinely thawed, cultured and passaged. Actively growing cells were divided into batches. Cells in the control group were grown under 5% CO2 + 20% O-2, and those in the hypoxia group were grown under 5% CO2 + 1% O-2. The experiments were performed after 12, 24 and 72 h under each growth condition. The percentages of CD13(+) cells were detected by flow cytometry, the expression of HIF-1 alpha and VEGF was detected by western blot analysis, the cell proliferation rate was detected by the MTT assay, and the apoptotic rate was detected by flow cytometry. The results showed that the percentage of CD133(+) cells, and the expressions of HIF-1 alpha and VEGF for the cells in the hypoxia group increased gradually from 12 to 24, to 72 h, while there were no equivalent changes in the control group. Cell proliferation in the two groups increased gradually from 12 to 24, to 72 h, but was significantly higher at all time-points in the hypoxia group (p< 0.05). There was no significant difference in terms of the amount of apoptotic cells at any of the three different time-points in either group, but the apoptotic cells in the hypoxia group were significantly less than those in the control group at each time-point, and the difference was statistically significant (p< 0.05). We conclude that the expression of CD133(+), HIF-1 alpha and VEGF in human prostate cancer cells is related to conditions of hypoxia, which ultimately promotes the proliferation and reduces apoptosis in these cells.
[1]Jilin Univ, Hosp 1, Dept Androl, 71 Xinmin St, Changchun 130021, Jilin, Peoples R China.
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