BKCa is a large conductance calcium activated potassium channel ubiquitously expressed in various cell types. Accumulating evidence demonstrates that BKCa is aberrantly expressed in many malignancies, involving in cancerous behaviors such as cell proliferation and migration. In this study, we investigated the functional role of BKCa in endometrial cancer HEC-1-B cells. Overexpression of BKCa by plasmid transfection enhanced endometrial cancer cell proliferation and migration. Conversely, silence of BKCa by lentivirus mediated RNAi system not only inhibited proliferation and migration but also impaired tumor growth in vivo. Patch clamp assay identified the BKCa currents in HEC-1-B cells, which was supported by the observation of channel activation or inhibition in response to the specific opener (NS1619) or blocker (IBTX) of BKCa. Moreover, NS1619 significantly increased cell proliferation and migration while IBTX exhibited the opposite effects. In summary, these data suggested an important role of BKCa in proliferation and migration of endometrial cancer HEC-1-B cells. Thus, BKCa may be established as a potential therapeutic target in endometrial cancer.
[1]Jilin Univ, Affiliated Hosp 1, Dept Gynecol & Obstet, Changchun 130021, Jilin, Peoples R China.
[2]Gen Hosp Shenyang Mil Area Command, Dept Oncol, 83 Wenhua Rd, Shenyang 110840, Liaoning, Peoples R China.
[3]PLA Army Gen Hosp, Affiliated Bayi Brain Hosp, Beijing 100700, Peoples R China.
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