This study aims to explore the effects of the TLR4 signaling pathway on the apoptosis of neuronal cells in rats with diabetes mellitus complicated with cerebral infarction ( DMCI). A DMCI model was established with 40 Sprague Dawley rats, which were assigned into blank, sham, DM + middle cerebral artery occlusion ( MCAO) and DM + MCAO + TAK242 groups. Superoxide dismutase ( SOD) activity and malondialdehyde ( MDA) content were measured. A TUNEL assay was applied for detecting cell apoptosis, and Western blotting was used for detecting the expression of TLR4, TNF- alpha, IL- 1 beta and apoptosis-related proteins. Compared with the blank and sham groups, there was an increase in cell apoptosis, expression of Bcl-2, Bax, cleaved caspase-3, TNF-alpha IL-1 beta and TLR4 proteins and MDA content and a decrease in SOD activity in the DM + MCAO and DM + MCAO + TAK242 groups. Compared with those in the DM + MCAO group, rats in the DM + MCAO + TAK242 group exhibited an increase in SOD activity and a decrease in cell apoptosis, expression of Bcl-2, Bax, cleaved caspase-3, TNF-alpha, IL-beta and TLR4 proteins and MDA content. Inhibition of the TLR4 signaling pathway reduces neuronal cell apoptosis and nerve injury to protect the brain.
[1]Jilin Univ, Hosp 1, Dept Neurol, Changchun 130021, Peoples R China.
[2]Jilin Univ, Hosp 1, Dept Infect Dis, Changchun 130021, Peoples R China.
[3]Jilin Univ, Hosp 1, Dept Radiol, Changchun 130021, Peoples R China.
[4]Jilin Univ, Hosp 1, Dept Neurosurg, Changchun 130021, Peoples R China.
(8.0+极速模式)