首页 / 院系成果 / 成果详情页

Inhibition of C-X-C Motif Chemokine 10 (CXCL10) Protects Mice from Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease 期刊论文

编号：

94f70f4b-1027-427c-8263-b7697ee4ddbc
作者：

Jing, Hongyu(井弘宇)#^[1]Liu, Lingyun^[2];Zhou, Junfeng(周俊峰)^[3]Yao, Hanxin(姚瀚鑫)*^[4]
语种：

English
期刊：

MEDICAL SCIENCE MONITOR ISSN：1643-3750 2018 年 24 卷 (5748 - 5753) ; AUG 17
收录：
关键词：

Chemokine CXCL10 Lung Diseases, Obstructive Smoke
摘要：

Background: Chronic obstructive pulmonary disease (COPD) is a type of obstructive lung disease characterized by long-term breathing problems and poor airflow. COPD can progress to persistent decline of pulmonary function. This study explored the effect of CXCL10 on COPD induced by cigarette smoke (CS) and its underlying mechanism. Material/Methods: Wild-type (WT) mice were randomly assigned into 3 groups: the control group, the CS group, and the intervention group. Mice in the CS group were exposed to CS and mice in the CXCL10 group were exposed to CS and CXCL10 neutralizing antibody. At 24 h after the last CS exposure, body weight and lung functions of each mouse were recorded. Mice were then anesthetized for collecting bronchoalveolar lavage fluid (BALF) and lung tissues. Levels of interleukin-6 (IL-6), keratinocyte chemotactic factor (KC), and monocyte chemoattractant protein-1 (MCP-1) in supernatant and lung homogenate were detected by ELISA and real-time PCR (RT-PCR), respectively. For in vitro experiments, human bronchial epithelial cells 16HBE were stimulated with different concentrations of cigarette smoke extract (CSE) and CXCL10. Cell viability and levels of inflammatory cytokines in the cell supernatant were detected by Cell Counting Kit-8 (CCK-8) and ELISA assay, respectively. Results: Our data showed significant weight loss and reduction of lung functions in mice in the CS group compared with those in the control group and intervention group. Increased levels of IL-6, KC, and MCP-1 in BALF and lung homogenate were observed in mice in the model group compared to those in the control group and intervention group. In vitro experiments also confirmed that CXCL10-neutralizing antibody can inhibit CSE-induced cell necrosis and activation of inflammatory cytokines. Conclusions: Inhibited CXCL10 protects against COPD progression by decreasing secretion of inflammatory factors, which provides a new direction for the clinical prevention and treatment of COPD.
推荐引用方式
GB/T 7714：

Jing Hongyu,Liu Lingyun,Zhou Junfeng, et al. Inhibition of C-X-C Motif Chemokine 10 (CXCL10) Protects Mice from Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease [J].MEDICAL SCIENCE MONITOR,2018,24:5748-5753.
APA：

Jing Hongyu,Liu Lingyun,Zhou Junfeng,Yao Hanxin.(2018).Inhibition of C-X-C Motif Chemokine 10 (CXCL10) Protects Mice from Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease .MEDICAL SCIENCE MONITOR,24:5748-5753.
MLA：

Jing Hongyu, et al. "Inhibition of C-X-C Motif Chemokine 10 (CXCL10) Protects Mice from Cigarette Smoke-Induced Chronic Obstructive Pulmonary Disease" .MEDICAL SCIENCE MONITOR 24(2018):5748-5753.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：96  下载次数：0

分享到：

浏览次数：96

下载次数：0

打印次数：0