首页 / 院系成果 / 成果详情页

Dracorhodin Perchlorate Induced Human Breast Cancer MCF-7 Apoptosis through Mitochondrial Pathways 期刊论文

编号：

9515faa8-9348-46e2-a15f-b80fe6297f0a
作者：

Yu, Jinghua(于静华)#^[1]Zheng, Guibin(郑桂彬)#^[2]Liu, Chunyu#^[3]Zhang, Liying^[4];Gao, Hongmei^[5];Zhang, Yahong(张亚宏)^[6]Dai, Chunyan^[1];Huang, Lin^[7];Meng, Xianying(孟宪瑛)*^[2]Zhang, Wenyan(张文艳)*^[1]Yu, Xiaofang(于晓方)*^[1]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF MEDICAL SCIENCES ISSN：1449-1907 2013 年 10 卷 9 期 (1149 - 1156) ; 2013
收录：
关键词：

dracorhodin perchlorate apoptosis mitochondrial pathway
摘要：

Objective: Dracorhodin perchlorate (DP) was a synthetic analogue of the antimicrobial anthocyanin red pigment dracorhodin. It was reported that DP could induce apoptosis in human prostate cancer, human gastric tumor cells and human melanoma, but the cytotoxic effect of DP on human breast cancer was not investigated. This study would investigate whether DP was a candidate chemical of anti-human breast cancer.
Methods: The MTT assay reflected the number of viable cells through measuring the activity of cellular enzymes. Phase contrast microscopy visualized cell morphology. Fluorescence microscopy detected nuclear fragmentation after Hoechst 33258 staining. Flowcytometric analysis of Annexin V-PI staining and Rodamine 123 staining was used to detect cell apoptosis and mitochondrial membrane potential (MMP). Real time PCR detected mRNA level. Western blot examined protein expression.
Results: DP dose and time-dependently inhibited the growth of MCF-7 cells. DP inhibited MCF-7 cell growth through apoptosis. DP regulated the expression of Bcl-2 and Bax, which were mitochondrial pathway proteins, to decrease MMP, and DP promoted the transcription of Bax and inhibited Bcl-2. Apoptosis-inducing factor (AIF) and cytochrome c which localized in mitochondrial in physiological condition were released into cytoplasm when MMP was decreased. DP activated caspase-9, which was the downstream of mitochondrial pathway. Therefore DP decreased MMP to release AIF and cytochrome c into cytoplasm, further activating caspase 9, lastly led to apoptosis.
Conclusion: Therefore DP was a candidate for anti-breast cancer, DP induced apoptosis of MCF-7 through mitochondrial pathway.
推荐引用方式
GB/T 7714：

Yu Jing-hua,Zheng Gui-bin,Liu Chun-yu, et al. Dracorhodin Perchlorate Induced Human Breast Cancer MCF-7 Apoptosis through Mitochondrial Pathways [J].INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,2013,10(9):1149-1156.
APA：

Yu Jing-hua,Zheng Gui-bin,Liu Chun-yu,Zhang Li-ying,&Yu Xiao-fang.(2013).Dracorhodin Perchlorate Induced Human Breast Cancer MCF-7 Apoptosis through Mitochondrial Pathways .INTERNATIONAL JOURNAL OF MEDICAL SCIENCES,10(9):1149-1156.
MLA：

Yu Jing-hua, et al. "Dracorhodin Perchlorate Induced Human Breast Cancer MCF-7 Apoptosis through Mitochondrial Pathways" .INTERNATIONAL JOURNAL OF MEDICAL SCIENCES 10,9(2013):1149-1156.
入库时间：

12/16/2019 3:18:16 PM
更新时间：

12/16/2019 3:18:16 PM
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：12  下载次数：0

分享到：

浏览次数：12

下载次数：0

打印次数：0