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Exposure to PM2.5 induces aberrant activation of NF-kappa B in human airway epithelial cells by downregulating miR-331 expression 期刊论文

编号：

a280191a-cc79-4b6c-a2ad-27e8dbd343b3
作者：

Song, Lei(宋磊)#^[1]Li, Dan(李丹)^[1]Li, Xiaoping(李小平)^[2]Ma, Lianjun^[3];Bai, Xiaoxue(白晓雪)^[4]Wen, Zhongmei(温中梅)^[1]Zhang, Xiufang^[1];Chen, Dong^[1];Peng, Liping(彭丽萍)*^[1]
语种：

英文
期刊：

ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY ISSN：1382-6689 2017 年 50 卷 (192 - 199) ; MAR
收录：
关键词：

PM2.5 microRNA Reactive oxygen species Lipopolysaccharide NF-kappa B Inflammation
摘要：

Exposure to particulate matter (PM) with an aerodynamic diameter <2.5 pari (PM2.5) induces reactive oxygen species (ROS) and pro-inflammatory cytokine production, leading to airway epithelial injury. However, the mechanisms underlying the toxicity of PM2.5 have not been clarified. Here, we show that exposure to PM2.5 induces sustained activation of the nuclear factor kappa B (NF-KB) signaling in human airway epithelial Beas-2B (B2B) cells. In addition, PM2.5 exposure significantly decreased miR-331 expression in B2B cells, which was abrogated by inhibition of ROS or phosphoinositide 3-kinase (PI3K)/Akt pathway. Induction of miR-331 overexpression attenuated the PM2.5 exposure-induced NFkBp65 nuclear translocation, IL-6 and IL-8 expression in B2B cells. Furthermore, miR-331 targeted the inhibitor of NF-KB Idnase beta (IKK-(3) by down-regulating the IKK-I3-regulated luciferase activity in HEK293 cells. Moreover, induction of miR-331 over-expression inhibited IKK-f3 expression while induction of IKK-13 over-expression prevented the inhibition of miR-331 on the PM2.5 exposure-induced NF-kBp65 nuclear translocation, IL-6 and IL-8 expression in B2B cells. Therefore, PM2.5 exposure decreased miR-331 expression via the ROS/PI3K/Akt pathway, resulting in an increase in the IKK-I3 expression and sustained NF-KB activation in human airway epithelial cells. Our findings may provide new insights into the molecular mechanisms underlying the toxicity of PM2.5 exposure and aid in design of new therapeutic strategies to prevent PM2.5-induced toxicity. (C) 2017 Elsevier B.V. All rights reserved.
推荐引用方式
GB/T 7714：

Song Lei,Li Dan,Li Xiaoping, et al. Exposure to PM2.5 induces aberrant activation of NF-kappa B in human airway epithelial cells by downregulating miR-331 expression [J].ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,2017,50:192-199.
APA：

Song Lei,Li Dan,Li Xiaoping,Ma Lianjun,&Peng Liping.(2017).Exposure to PM2.5 induces aberrant activation of NF-kappa B in human airway epithelial cells by downregulating miR-331 expression .ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY,50:192-199.
MLA：

Song Lei, et al. "Exposure to PM2.5 induces aberrant activation of NF-kappa B in human airway epithelial cells by downregulating miR-331 expression" .ENVIRONMENTAL TOXICOLOGY AND PHARMACOLOGY 50(2017):192-199.
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