[1]Department of Neurology, The First Hospital of Jilin University, Changchun 130021, China.
[2]Clinical Laboratory, The Affiliated Hospital of Changchun University of Chinese Medicine, Changchun 130021, China.
[3]Department of Endoscopy Center, The China-Japan Union Hospital of Jilin University, Changchun 130033, China.
[4]Department of Cadre"s Ward, The First Hospital of Jilin University, Changchun 130021, China.
[5]Department of Respiratory Medicine, The First Hospital of Jilin University, Changchun 130021, China.
The demand for the chemopreventive drug from the plant source is increasing in recent times, owing to its various biological activities without any adverse effect. The intention of this current study was to examine the anti-glioma effect of Withaferin A (WFA) on C6 glioma cell line model.;C6 glioma cells were administrated with different concentration of WFA (50, 100, 200 and 500 μg/mL) and DMSO (control) group to examine its anti-proliferative, anti-inflammatory and pro-apoptotic activities.;Treatment with WFA showed a significant decline in the glioma cell count in a dose-dependent manner and thus proving its anti-proliferative effect. Similarly, inflammatory markers were also substantially lowered upon treatment with different concentration of WFA. However, DNA fragmentation and apoptotic markers like Caspase-3 and 9 were concomitantly enhanced after co-cultured with different concentration of WFA and thus exhibiting its cytotoxicity efficacy. Furthermore, the protein expression of Bcl2 and Bax were markedly downregulated and upregulated respectively; upon treatment with WFA on C6 glioma cells.;The outcome of this study evidently demonstrates that C6 glioma cells co-cultured with increased concentration of WFA, showed an anti-proliferative, anti-inflammatory and pro-apoptotic effect in a dose-dependent fashion.;
(8.0+极速模式)