首页 / 院系成果 / 成果详情页

Antitumor activity of a novel survivin siRNA 期刊论文

编号：

b5e6d9e6-a5d8-4ef6-b36d-b93201dd4b2e
作者：

Li, Yuhuan#^[1]Li, Yujing^[1];Lee, Robert J.^[1,2];Wang, Xi^[1];Du, Lin^[3];Zhao, Lingzhi^[4];Liu, Yan^[1];Teng, Lesheng(滕乐生)*^[1]
语种：

英文
期刊：

PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES ISSN：1011-601X 2015 年 28 卷 5 期 (1887 - 1890) ; SEP
收录：
关键词：

Survivin siRNA breast cancer cell proliferation cell cycle
摘要：

Breast cancer resistance to therapy can result from expression of antiapoptotic genes. Survivin is an antiapoptotic gene that is over expressed in most human tumors. RNA interference using short interfering RNA (siRNA) can be used to specifically inhibit survivin expression. A novel siRNA targeting survivin was used to process MCF-7 cells. Cellular survivin mRNA and protein levels were determined by real-time qRT-PCR and Western blot, respectively. Cellular morphology and cell cycle were determined by fluorescence microscopy and flow cytometry. Cell proliferation was measured by MTT assay. Our data showed that the novel survivin-targeted siRNA could efficiently knockdown the expression of survivin, inhibit cell proliferation and cell cycle, egpecially at the G2/M checkpoint. These data suggest that the siRNA has potential for therapeutic applications.
推荐引用方式
GB/T 7714：

Li Yuhuan,Li Yujing,Lee Robert J., et al. Antitumor activity of a novel survivin siRNA [J].PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,2015,28(5):1887-1890.
APA：

Li Yuhuan,Li Yujing,Lee Robert J.,Wang Xi,&Teng Lesheng.(2015).Antitumor activity of a novel survivin siRNA .PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES,28(5):1887-1890.
MLA：

Li Yuhuan, et al. "Antitumor activity of a novel survivin siRNA" .PAKISTAN JOURNAL OF PHARMACEUTICAL SCIENCES 28,5(2015):1887-1890.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：32  下载次数：0

分享到：

浏览次数：32

下载次数：0

打印次数：0