The determination of Prostaglandin (PG) E-1 in plasma is challenged by its low concentration (pg/mL) and endogenous interference. An LC-MS/MS method for the determination of PGE(1) in dog plasma has been developed and validated. Plasma being sampled at 4 degrees C and treated with indomethacin effectively inhibited interferents synthesized post-sampling. Samples were subjected to one-step extraction and separated by reversed phase HPLC with a short cycle time of 3 min. An LLOQ of 10 pg/mL was achieved with 500 mu l plasma. The method was applied to a pharmacokinetic study in beagle dogs involving an intravenous infusion of 3.2 mu g/kg PGE(1). The half-life was recovered at 7 min. The simple, sensitive and rapid method was suitable to be applied to pharmacokinetic studies of PGE(1) at clinically relevant doses. (c) 2013 Elsevier B.V. All rights reserved.
[1]Jilin Univ, Coll Life Sci, Changchun 130012, Peoples R China.
[2]Jilin Univ, Sch Pharmaceut Sci, Changchun 130021, Peoples R China.
[3]Jilin Univ, Bethune Hosp 1, Clin Pharmacol Ctr, Res Inst Translat Med, Changchun 130061, Peoples R China.
[4]Jilin Univ, Res Ctr Drug Metab, Changchun 130012, Peoples R China.
[5]Jilin Univ, Sch Life Sci, Natl Engn Lab AIDS Vaccine, Changchun 130012, Jilin Province, Peoples R China.
[6]Jilin Univ, Sch Life Sci, Minist Educ, Key Lab Mol Enzymol & Engn, Changchun 130012, Jilin Province, Peoples R China.
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