The aim of the present study was to identify and characterize side population (SP) cells in primary cervical cancer. A primary culture was successfully established, and the SP cells were isolated via fluorescence-activated cell sorting. Subsequently, in vitro analysis of clonogenic capacity by soft agar assay and in vivo analysis of tumorigenicity were performed. The isolated SP cells accounted for similar to 4.73% of the total primary culture cells. The SP cells had a decreased proliferation rate and an increased distribution in G(0)/G(1) compared with non-SP (NSP) cells. Following isolation, SP cells exhibited increased proliferative and self-renewal potency compared with NSP cells. Furthermore, significant ATP binding cassette subfamily G member 2 (ABCG2) expression was detected in SP cells but not in NSP cells. The tumor formation rate of SP cells was longer, and the tumor size and tumor formation rate of SP cells were increased in non-obese diabetic/severe combined immunodeficiency mice. In conclusion, the present study demonstrated that SP cells can be isolated from primary cervical cancer cell culture, and SP cells are enriched with stem cell-like cells that have a high capacity for colony formatiort, and tumorigenesis.
[1]Jilin Univ, Hosp 1, Dept Gynecol Oncol, 71 Xinmin Ave, Changchun 130021, Jilin, Peoples R China.
[2]Jilin Univ, Hosp 1, Prenatal Diag Ctr, Changchun 130021, Jilin, Peoples R China.
[3]Jilin Univ, Hosp 1, Genet Diag Ctr, Changchun 130021, Jilin, Peoples R China.
[4]Acad Mil Med Sci, Mil Vet Inst, Chinese Peoples Liberat Army, Genet Engn Lab, Changchun 130122, Jilin, Peoples R China.
(8.0+极速模式)