The present investigation was undertaken to evaluate the potential biochemical changes associated with renal parenchyma 6-12 months after renal transplantation and delayed graft failure. Serumconcentrations of transforming growth factor-beta these subjects always remained elevated. In addition, examination of tissue from needle biopsies confirmed that there were consistent changes in the enzyme lysyl oxidase, which functions as an amine oxidase, modifies lysine residues on collagen and cross-links in a process of modulation of the extracellular matrix. Parenchymal levels of hypoxia-inducible factor-1 alpha and fibronectin were elevated, as detected by Western blotting. These findings indicate an ongoing ischaemic insult, which might result from increased tissue fibrosis or, in some cases, might be additive with pre-existing pathophysiological factors that constrain proper renal haemodynamics. Thus, increased lysyl oxidase activity, which we assayed, is a potential unfavourable mechanism occurring in these kidneys that are undergoing failure and probably causes increased fibrosis within the organ and causes ischaemia, renovascular hypertension and a cascade leading to renal dysfunction and failure.
[1]Jilin Univ, First Hosp, Dept Nephrol, No. 71,Xinmin St, Changchun 130021, Kagawa, Peoples R China.
[2]Jilin Univ, First Hosp, Dept Gynecol, Changchun 130021, Kagawa, Peoples R China.
[3]Jilin Univ, First Hosp, Dept Obstet, Changchun 130021, Kagawa, Peoples R China.
[4]Univ Iowa Carver, Coll Med, Holden Comprehens Canc, Dept Mol Physiol & Biophys, Iowa City, IA 52242 USA.
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