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A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer 期刊论文

编号：

c427378a-dafc-47d4-b24f-ec84e1c9a6dd
作者：

Dong, Mengmeng#^[1,2]Chen, Jiawen#^[2]Zhang, Jiayuan^[2];Liang, Xiao^[2];Yang, Jiebing^[2];Li, Dan(李丹)*^[1]Li, Quanshun(李全顺)*^[2]
语种：

英文
期刊：

INTERNATIONAL JOURNAL OF NANOMEDICINE ISSN：1178-2013 2019 年 14 卷 (1149 - 1161)
收录：
关键词：

cholesterol-g-poly(amine-co-ester) p53 gene delivery gene therapy anti-proliferation effect anti-migration effect
摘要：

Background: An amphiphilic cationic copolymer cholesterol-g-poly(amine-co-ester), namely Chol-g-PMSC-PPDL synthesized in a chemoenzymatic route has been utilized as a carrier for p53 gene delivery to check its antitumor efficacy, using human prostate cancer cell line PC-3 (p53 null) as a model.
Materials and methods: The transfection efficiency was measured by quantitative PCR and Western blotting assay. The anti-proliferative effect was detected using MTT method, colony formation assay and Live/Dead staining. The anti-migration effect was evaluated through wound healing and Transwell migration assays.
Results: The transfection efficiency assay indicated that the carrier-mediated p53 gene transfection could dramatically enhance the intracellular p53 expression level. Through p53 gene delivery, obvious anti-proliferative effect could be detected which was elucidated to be associated with the simultaneous activation of mitochondrial-dependent apoptosis pathway and cell cycle arrest at G1 phase. Meanwhile, the anti-migration effect could be obtained after p53 gene transfection.
Conclusion: Chol-g-PMSC-PPDL-mediated p53 gene transfection could potentially be employed as a promising strategy for achieving effective anti-tumor response.
推荐引用方式
GB/T 7714：

Dong Mengmeng,Chen Jiawen,Zhang Jiayuan, et al. A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer [J].INTERNATIONAL JOURNAL OF NANOMEDICINE,2019,14:1149-1161.
APA：

Dong Mengmeng,Chen Jiawen,Zhang Jiayuan,Liang Xiao,&Li Quanshun.(2019).A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer .INTERNATIONAL JOURNAL OF NANOMEDICINE,14:1149-1161.
MLA：

Dong Mengmeng, et al. "A chemoenzymatically synthesized cholesterol-g-poly(amine-co-ester)-mediated p53 gene delivery for achieving antitumor efficacy in prostate cancer" .INTERNATIONAL JOURNAL OF NANOMEDICINE 14(2019):1149-1161.
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