Accurate regulation of cellular zinc signaling is imperative to decipher underlying zinc functions and develop new therapeutic agents. However, the ability to modulate zinc in a spatiotemporal manner remains elusive. We herein report an intelligent spiropyran-upconversion (SP-UCNPs) based nanosystem that enables near-infrared (NIR) light-controlled zinc release at precise times and locations. The magnitude of zinc release can be simply manipulated by varying the duration of NIR irradiation. Moreover, the utilization of NIR light not only showed little damage to cells but also significantly improved penetration depth. By evaluating activity of a model protein, phosphatase 2A, we further validated zinc signaling activation. Importantly, our strategy may be broadly applicable to other types of metal ions, like the ubiquitous second messenger calcium. More importantly, our strategy can potentially enable the precise control of specific signaling pathways of metal ions while minimizing cellular damage, facilitating the advanced manipulation of cellular dynamics.
[1]Chinese Acad Sci, Changchun Inst Appl Chem, Lab Chem Biol, Changchun 130022, Jilin, Peoples R China.
[2]Chinese Acad Sci, Changchun Inst Appl Chem, State Key Lab Rare Earth Resources Utilizat, Changchun 130022, Jilin, Peoples R China.
[3]Univ Chinese Acad Sci, Beijing 100039, Peoples R China.
[4]Jilin Univ, Affiliated Hosp 1, Stem Cell & Canc Ctr, Changchun 130061, Jilin, Peoples R China.
(8.0+极速模式)