Objectives The drawback of bleeding caused by chronic antiplatelet therapy is persecuting patients with thrombotic diseases severely. Based on the dual-directional regulatory effect of Panax notoginseng on platelet, the present study focused on the effect of Notoginsenoside Ft1, a saponin with effect in promoting platelet aggregation. Key findings According to the present study, Notoginsenoside Ft1 cannot stimulate platelet aggregation independently. However, the effect in enhancing aggregation induced by thrombin, collagen and ADP is peaked at 5-10 mu m. In addition, thrombin-induced activation of PLC gamma 2-IP3/DAG-[Ca2+]/PKC-TXA(2) signalling was potentiated by Notoginsenoside Ft1, as well. Furthermore, the mice tail bleeding time was shortened by administration of Notoginsenoside Ft1 significantly. And the bleeding time prolonged by aspirin was also restored by Ft1. Conclusions The haemostatic effect of Notoginsenoside Ft1 was exerted through potentiation of PLC gamma 2-IP3/DAG-[Ca2+]/PKC-TXA(2) signalling pathway stimulated by other stimulators. Notoginsenoside Ft1 has the potential to be developed into supplements in antiplatelet therapy to prevent the drawback of bleeding.
[1]Northwest A&F Univ, Coll Vet Med, Yangling 712100, Shaanxi, Peoples R China.
[2]Jilin Univ, Hosp 1, Dept Neurosurg, Changchun, Jilin, Peoples R China.
[3]Shenyang Pharmaceut Univ, Sch Pharm, Shenyang, Liaoning, Peoples R China.
(8.0+极速模式)