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Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132) 期刊论文

编号：

dac998cd-5b18-49b6-8911-bdfb1d766c33
作者：

Zhang, Kewei(张科伟)#^[1]Li, Yang(李洋)#^[2]Qu, Limei(曲丽梅)^[3]Ma, Xiaobo(马小波)^[3]Zhao, Hongguang(赵红光)*^[4]Tang, Ying(唐颖)*^[2]
语种：

英文
期刊：

ONCOTARGETS AND THERAPY ISSN：1178-6930 2018 年 11 卷 (5269 - 5278)
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关键词：

non-small-cell lung cancer SOX2OT miR-132 ZEB2
摘要：

Background: Long noncoding RNA (lncRNA) Sox2 overlapping transcript (SOX2OT) has been reported to be upregulated in various types of cancers, including non-small-cell lung cancer (NSCLC). However, the biological role and underlying mechanism of SOX2OT activity in NSCLC remain largely unknown. This study aims to investigate the function and possible molecular mechanisms of SOX2OT in NSCLC.
Materials and methods: Quantitative real-time polymerase chain reaction was used to detect SOX2OT expression, and cellular proliferation, migration, and invasion were measured using cell counting kit-8, wound healing, and Transwell invasion assays, respectively. Western blotting was used to determine protein expression. Starbase 2.0 and luciferase reporter assay were utilized to identify the molecular target of SOX2OT.
Results: Here, we discovered that SOX2OT was markedly upregulated in NSCLC tissues and cell lines. Knockdown of SOX2OT inhibited the proliferation, migration, invasion, and epithelial-mesenchymal transition (EMT) process in NSCLC cells. Moreover, we explored the regulatory mechanism of SOX2OT and found that SOX2OT directly bound micro RNA 132 (miR-132) in NSCLC cells. Importantly, miR-132 inhibition partially reversed the SOX2OT knockdown-mediated inhibitory effect on cell proliferation, migration, invasion, and EMT process. We also found that SOX2OT could regulate zinc finger E-box-binding homeobox 2 (a target of miR-132) expression, which played crucial roles in tumor cell proliferation and invasion.
Conclusion: These findings indicated that SOX2OT was a noncoding oncogene that exerted important regulatory functions in NSCLC via sponging miR-132 and might represent a novel strategy for overcoming this disease.
推荐引用方式
GB/T 7714：

Zhang Kewei,Li Yang,Qu Limei, et al. Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132) [J].ONCOTARGETS AND THERAPY,2018,11:5269-5278.
APA：

Zhang Kewei,Li Yang,Qu Limei,Ma Xiaobo,&Tang Ying.(2018).Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132) .ONCOTARGETS AND THERAPY,11:5269-5278.
MLA：

Zhang Kewei, et al. "Long noncoding RNA Sox2 overlapping transcript (SOX2OT) promotes non-small-cell lung cancer migration and invasion via sponging microRNA 132 (miR-132)" .ONCOTARGETS AND THERAPY 11(2018):5269-5278.
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