Background: Increased inflammation was found in the left stellate ganglion (LSG) in patientswith malignant ventricular arrhythmia (VA). However, the role of inflammation in LSG remodeling is unknown. This study aimed to investigate whether exogenous interleukin-1 beta (IL-1 beta) could aggravate VA through regulating LSG remodeling.
Methods: Twenty-four canines who received saline (Control, n = 8), IL-1 beta injection into the LSG (n = 8) or IL-1 receptor antagonist (IL-Ra) pre-injection plus IL-1 beta injection (n = 8) were included. Ventricular electrophysiology parameters, heart rate variability (HRV), LSG activity were measured at different time points. VA was recorded for 60 min after ischemia and then LSG tissues were collected for molecular detection.
Results: Compared with the control group, IL-1 beta injection decreased the effective refractory period, action potential duration (APD) 90 and increased the maximal slope of the restitution curve in normal hearts. Besides, the occurrence of VA was significantly increased in the IL-1 beta group. Additionally, IL-1 beta injection increased the sympathetic indices of HRV and LSG activity in normal and ischemic hearts. Mechanically, the mRNA expression of pro-inflammatory cytokines and protein expression of c-fos, nerve growth factor and neuropeptide Y in LSG were increased, whereas the expression of neuronal nitric oxide synthase was decreased in the IL-1 beta group. More importantly, all these effects induced by IL-1 beta were attenuated by IL-1Ra pre-injection.
Conclusion: Increased inflammation induced by IL-1 beta injection aggravates ischemia induced VA through regulating the neuronal remodeling of the LSG. Inflammation induced neuroplasticity may be a novel mechanism and therapeutic target for VA. (C) 2017 Published by Elsevier Ireland Ltd.
[1]Wuhan Univ, Dept Cardiol, Renmin Hosp, Cardiovasc Res Inst,Hubei Key Lab Cardiol, Wuhan, Peoples R China.
[2]Jilin Univ, Hosp 1, Dept Cardiol, Changchun, Jilin, Peoples R China.
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