To explore an optimal frequency of whole-body low-dose radiation (LDR) to protect the kidney from diabetes, type 1 diabetic mice were induced with multiple injections of low-dose streptozotocin in male C57BL/6J mice. Diabetic or age-matched normal mice received whole-body exposure to 12.5 or 25 mGy either every other day or weekly for 4 or 8 weeks. Diabetes decreased the urinary creatinine and increased the microalbumin in urine, renal accumulation of 3-nitrotyrosine and 4-hydroxynonenal, and renal expression of collagen IV and fibronectin. All these renal pathological and functional changes in diabetic mice were significantly attenuated by exposure to LDR at all regimens. However, whole-body exposure of diabetic mice to 25 mGy weekly and to 12.5 mGy every other day for 8 weeks provided a better prevention of diabetic nephropathy than other LDR regimens. Furthermore, whole-body exposure to 25 mGy weekly for 8 weeks showed no detectable effect on the kidney of normal mice, but whole-body exposure to normal mice at 12.5 mGy every other day for 8 weeks increased urinary microalbumin and renal expression of collagen IV and fibronectin. These results suggest that whole-body exposure to LDR at 25 mGy weekly is the optimal condition of LDR to protect the kidney from diabetes.
[1]Shanghai Jiao Tong Univ, Tongren Hosp, Dept Endocrinol, Sch Med, Shanghai, Peoples R China.
[2]Univ Louisville, Dept Pediat, Pediat Res Inst, Louisville, KY 40292 USA.
[3]PLA Rocket Force, Gen Hosp, Beijing, Peoples R China.
[4]Jilin Univ, Hosp 1, Changchun, Jilin, Peoples R China.
[5]Univ Louisville, Dept Radiat Oncol, Louisville, KY 40292 USA.
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