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Phloretin Attenuates Listeria monocytogenes Virulence Both In vitro and In vivo by Simultaneously Targeting Listeriolysin O and Sortase A 期刊论文

编号：

de629e92-cb49-40fa-9866-90e1db116438
作者：

Wang, Jianfeng(王建锋)#^[1,2,3]Liu, Bowen#^[1,2,3]Teng, Zihao^[1,2,3];Zhou, Xuan^[1,2,3];Wang, Xiyan^[1,2,3];Zhang, Bing^[1,2,3];Lu, Gejin^[1,2,3];Niu, Xiaodi^[1,2,3];Yang, Yongjun*^[1,2,3]Deng, Xuming(邓旭明)*^[1,2,3]
语种：

English
期刊：

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY ISSN：2235-2988 2017 年 7 卷 ; JAN 19
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关键词：

Listeria monocytogenes listeriolysin O sortase A phloretin anti-infective therapy
摘要：

The critical roles of sortase A (SrtA) and listeriolysin O (LLO) in Listeria monocytogenes pathogenicity render these two virulence factors as ideal targets for the development of anti-virulence agents against L. monocytogenes infection. Additionally, the structures of SrtA and LLO are highly conserved among the members of sortase enzyme family and cholesterol dependent toxin family. Here, phloretin, a natural polyphenolic compound derived from apples and pears that has little anti-L. monocytogenes activity, was identified to simultaneously inhibit LLO expression and neutralize SrtA catalytic activity. Phloretin neutralized SrtA activity by causing a conformational change in the protein's active pocket, which prevented engagement with its substrate. Treatment with phloretin simultaneously reduced L. monocytogenes invasion into host cells and blocked the escape of vacuole-entrapped L. monocytogenes into cytoplasm. Further, L. monocytogenes-infected mice that received phloretin showed lower mortality, decreased bacterial burden and reduced pathological injury. Our results demonstrate that phloretin is a promising anti-infective therapeutic for infections caused by L. monocytogenes due to its simultaneous targeting of SrtA and LLO, which may result in fewer side effects than those caused by other antibiotics.
推荐引用方式
GB/T 7714：

Wang Jianfeng,Liu Bowen,Teng Zihao, et al. Phloretin Attenuates Listeria monocytogenes Virulence Both In vitro and In vivo by Simultaneously Targeting Listeriolysin O and Sortase A [J].FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,2017,7.
APA：

Wang Jianfeng,Liu Bowen,Teng Zihao,Zhou Xuan,&Deng Xuming.(2017).Phloretin Attenuates Listeria monocytogenes Virulence Both In vitro and In vivo by Simultaneously Targeting Listeriolysin O and Sortase A .FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY,7.
MLA：

Wang Jianfeng, et al. "Phloretin Attenuates Listeria monocytogenes Virulence Both In vitro and In vivo by Simultaneously Targeting Listeriolysin O and Sortase A" .FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY 7(2017).
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