首页 / 院系成果 / 成果详情页

The Effects of Irreversible Electroporation on the Colon in a Porcine Model 期刊论文

编号：

deb8d6cb-5ee0-4f3f-9c7e-720c66e3f228
作者：

Luo, Xiaomei#^[1]Liang, Xianjun#^[2]Li, Jiannan^[3];Shi, Jian^[3];Zhang, Wenlong^[4];Chai, Wei(柴微)^[5]Wu, Jiuping^[6];Guo, Shuai^[7];Fang, Gang^[8];Zhou, Xulong^[9];Zhang, Jianhua^[10];Xu, Kecheng^[8];Zeng, Jianying^[11];Niu, Lizhi(牛立志)*^[8]
语种：

英文
期刊：

PLOS ONE ISSN：1932-6203 2016 年 11 卷 12 期 ; DEC 1
收录：
摘要：

Background and Aim
Irreversible electroporation (IRE) is a method of targeted cell ablation which has been suggested as a potential cancer therapy as it leaves structures such as blood vessels and the extracellular matrix intact, thereby allowing the rapid recovery of healthy tissue. Here, we investigated the effects of IRE on the colon in vivo in a porcine model.
Methods
IRE ablation was performed on the colon walls of 12 female Tibet mini-pigs, creating a total of 24 lesions. Lesions were monitored periodically by endoscopy. The pigs were euthanized 7, 14, 21 or 28 days after IRE ablation and the colons harvested for gross and histological analysis. Sections were stained with hematoxylin and eosin (H&E), Masson"s trichrome (MT) stain and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) assay.
Results
All pigs tolerated the ablation procedure without serious clinical symptoms or complications. There was no evidence of perforation by endoscopy or gross postmortem examination. All lesions were characterized by necrotic cell death with mild inflammation and hyperemia, with a sharp demarcation between ablated and adjacent normal tissue. A fibrous scar was observed in the ablated colon tissue. Histological analysis revealed damage to each layer of the colon. Histopathology findings also showed the preservation of extracellular structures and the recovery of the ablated colon.
Conclusions
The complete ablation of the target area, its rapid recovery and the lack of posttreatment symptoms suggest that IRE ablation may be a promising therapy for tumors located adjacent to or violating the colon wall.
推荐引用方式
GB/T 7714：

Luo Xiaomei,Liang Xianjun,Li Jiannan, et al. The Effects of Irreversible Electroporation on the Colon in a Porcine Model [J].PLOS ONE,2016,11(12).
APA：

Luo Xiaomei,Liang Xianjun,Li Jiannan,Shi Jian,&Niu Lizhi.(2016).The Effects of Irreversible Electroporation on the Colon in a Porcine Model .PLOS ONE,11(12).
MLA：

Luo Xiaomei, et al. "The Effects of Irreversible Electroporation on the Colon in a Porcine Model" .PLOS ONE 11,12(2016).
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：15  下载次数：0

分享到：

浏览次数：15

下载次数：0

打印次数：0