首页 / 院系成果 / 成果详情页

The screening and analysis of protein signatures and signaling associated with chemoresistance based on Protein Pathway Array technology in gastric cancer 期刊论文

编号：

e9ac512d-64cb-40ce-9283-57bea789858e
作者：

Lian, Guodong#^[1]Li, Leping^[1];Ye, Fei^[2];Wang, Daguang(王大广)^[3]Liu, Jinglei^[1];Shi, Yulong^[1];Jing, Changqing^[1];Suo, Jian^[3];Zhang, David Y.^[2];Chen, Man*^[4]
语种：

English
期刊：

ONCOLOGY REPORTS ISSN：1021-335X 2018 年 39 卷 1 期 (307 - 315) ; JAN
收录：
关键词：

chemoresistance gastric cancer Protein Pathway Array Ingenuity Pathway Analysis
摘要：

The present study was aimed to identify proteins associated with signaling pathways involved in chemoresistance, and establish a predictive model for chemoresistance in gastric cancer patients after radical surgery. A total of 140 clinically-staged III gastric cancer samples from patients after D2 radical gastrectomy were enrolled in the present study. Protein Pathway Array (PPA) and 286 antibodies were used to assess the protein expression in tumor tissues of patients. The Significance Analysis of Microarray (SAM) software and clustering and discriminant analysis were used to identify differentially expressed proteins between chemosensitive and chemoresistant subsets, and a predictive model for chemoresistance was established using the independent predictive factors. The Ingenuity Pathway Analysis (IPA) software was also used to investigate the relationship between proteins and the signaling transduction network. A total of 23 proteins were differentially expressed between 67 chemosensitive and 73 chemoresitant tumor tissues. Six proteins including PLK1 and DACH1 were independent risk factors for chemoresistance. A predictive model for chemoresistance by these proteins was established, and the accuracy, the sensitivity, and the specificity of this modal was 89.3, 90.3 and 88.2%, respectively. In addition, the present study revealed that differentially expressed proteins were closely related to cellular activity, DNA methylation and DNA damage and repair, and also involved in the ERK/MAPK, Wnt/beta-catenin, PI3K/AKT, apoptosis and p53 signaling pathways. In conclusion, the predictive model established by PPA may be an effective detection system for predicting the chemosensitivity of gastric cancer patients after D2 gastrectomy.
推荐引用方式
GB/T 7714：

Lian Guodong,Li Leping,Ye Fei, et al. The screening and analysis of protein signatures and signaling associated with chemoresistance based on Protein Pathway Array technology in gastric cancer [J].ONCOLOGY REPORTS,2018,39(1):307-315.
APA：

Lian Guodong,Li Leping,Ye Fei,Wang Daguang,&Chen Man.(2018).The screening and analysis of protein signatures and signaling associated with chemoresistance based on Protein Pathway Array technology in gastric cancer .ONCOLOGY REPORTS,39(1):307-315.
MLA：

Lian Guodong, et al. "The screening and analysis of protein signatures and signaling associated with chemoresistance based on Protein Pathway Array technology in gastric cancer" .ONCOLOGY REPORTS 39,1(2018):307-315.
条目包含文件：

文件类型：PDF,文件大小：

正在加载全文

未找到全文

浏览次数：14  下载次数：0

分享到：

浏览次数：14

下载次数：0

打印次数：0