To provide scientific data of the internal transcribed spacer (ITS) and chloroplast trnL-F sequences for identification of Potentilla bifurca L var. humilior Rupr., we extracted the genome DNA from the leaves of Potentilla bifurca L var. humilior Rupr. collected in Jilin Province, amplified the ITS and chloroplast trnL-F region using universal primers of angiosperm, and sequenced the purified PCR products directly. The obtained sequences were edited by Genetyx and reported here.