Glioma is a worldwide malignancy, which displays significantly active metastasis and angiogenesis. Interaction between long non-coding RNAs (lncRNAs) and microRNAs (miRNAs) has been shown to play crucial role in regulating tumor properties. However, the potential of lncRNA X-inactive specific transcript (XIST) to function as a miRNA regulator and its relevance in glioma tumorigenicity and angiogenesis have so far remained unclear. Expression analysis of lncRNA XIST in glioma cells revealed its significant up-regulation. Interestingly, silencing of XIST repressed both metastatic and pro-angiogenic ability in vitro as well as in vivo. Subsequent studies revealed that lncRNA XIST expression inversely correlated with miR-429 expression in glioma cells; miR-429 modulated XIST expression by directly targeting the XIST gene sequence. In addition, miR-429 inhibitor restored metastatic and pro-angiogenic ability of gliomas abolished by silencing XIST. Our data provide insight into the key roles of the lncRNA-miRNA functional network in gliomas, which can aid in developing new therapeutic strategies for gliomas through clinical trials.
[1]Jilin Univ, Hosp 1, Dept Vasc Surg, Changchun 130021, Jilin, Peoples R China.
[2]Jilin Univ, Norman Bethune Hlth Sci Ctr, Dept Clin Med, Changchun 130021, Jilin, Peoples R China.
[3]Jilin Univ, Hosp 1, Dept Pediat Emergency, Changchun 130021, Jilin, Peoples R China.
[4]Jilin Univ, Hosp 1, Dept Otolaryngol Head & Neck Surg, Changchun 130021, Jilin, Peoples R China.
[5]Chinese Med Univ, Coll Life Sci, Dept Cell Biol, Hangzhou 310053, Zhejiang, Peoples R China.
[6]Jilin Univ, Sch Pharmaceut Sci, Dept Regenerat Med, 1266 Fujin Rd, Changchun 130021, Jilin, Peoples R China.
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