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In vitro analysis of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells 期刊论文

编号：

fe0918a8-77f9-4535-977c-6cf57c843a0b
作者：

Niu, Chao(牛超)#^[1]Jin, Haofan(金浩范)#^[1]Li, Min(李敏)^[1]Xu, Jianting^[1];Xu, Dongsheng(徐东升)^[1]Hu, Jifan(胡继繁)^[1]He, Hua(何华)^[1]Li, Wei(李薇)*^[1]Cui, Jiuwei(崔久嵬)*^[1]
语种：

英文
期刊：

BMC IMMUNOLOGY ISSN：1471-2172 2015 年 16 卷 ; OCT 12
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关键词：

Natural killer cells Cytokine-induced killer cells Gamma-delta T cells Cytotoxicity
摘要：

Background: Recent studies have focused on the significant cytotoxicity of natural killer (NK) cells, cytokine-induced killer (CIK) cells, and gamma-delta (gamma delta) T cells in tumor cells. Nevertheless, the therapeutic features of these cell types have not been compared in the literature. The aim of this study was to evaluate the feasibility of activation and expansion of NK, CIK, and gamma delta T cells from cancer patients in vitro, and to clarify the differences in their antitumor capacities.
Methods: NK, CIK, and gamma delta T cells were induced from the peripheral blood mononuclear cells of 20 cancer patients by using specific cytokines. Expression of CD69, NKG2D, CD16, granzyme B, perforin, IFN-gamma, and IL-2 was measured by flow cytometry. Cytokine production and cytotoxicity were analyzed by enzyme-linked immunosorbent assay and Calcein-AM methods.
Results: NK cell proliferation was superior to that of CIK cells, but lower than that of gamma delta T cells. NK cells had a much stronger ability to secrete perforin, granzyme B, IFN-gamma, and IL-2 than did CIK and gamma delta T cells, and imparted significantly higher overall cytotoxicity.
Conclusions: Expanded NK cells from cancer patients are the most effective immune cells in the context of cytokine secretion and anti-tumor cytotoxicity in comparison to CIK and gamma delta T cells, making them an optimal candidate for adoptive cellular immunotherapy.
推荐引用方式
GB/T 7714：

Niu Chao,Jin Haofan,Li Min, et al. In vitro analysis of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells [J].BMC IMMUNOLOGY,2015,16.
APA：

Niu Chao,Jin Haofan,Li Min,Xu Jianting,&Cui Jiuwei.(2015).In vitro analysis of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells .BMC IMMUNOLOGY,16.
MLA：

Niu Chao, et al. "In vitro analysis of the proliferative capacity and cytotoxic effects of ex vivo induced natural killer cells, cytokine-induced killer cells, and gamma-delta T cells" .BMC IMMUNOLOGY 16(2015).
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